SPLIT INTEINS PROTEIN MODIFICATION
Splittera is a tool for site-specific protein modification, both in vivo and in vitro.
The use of ZIP's split inteins can form a covalent bond between a protein and virtually any kind of molecule, including other proteins, fluorescent probes, peptides, DNA or a chemical compound.
Splittera applications in bioconjugation:
- Conjugation of a fluorescent probe to the N- or C-terminus of a protein
- Generation of bispecific antibodies by conjugating two different Fabs to the Fc portion of an antibody in a fast and simple reaction
- Generation of large libraries of cyclic peptides for drug discovery
- Generation of high molecular weight protein-based polymers