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Lab 02 Akta


Splittera can be easily converted into a protein purification tool.

The purification system takes advantage of the great affinity existing between the two domains of a ZIP's split intein to separate the protein of interest (POI) from the other proteins of the expression host. Once the POI is bound to the column, a change in the reaction conditions triggers the intein cleavage, which releases a tag-less protein without any extra amino acid.

Splittera as a purification tool:

- independent of the first amino acid of the POI

- reaches very high yields of pure protein

- fast, single step process

- universal, equivalent to the Protein A system for antibody purification

- works with proteins expressed in the most commonly used expression hosts

- works with proteins expressed as inclusion bodies


Learn more in our Splittera Protein purification flyer